Bio
Education and Work Experiences:
17/10/11-15/04/12.
Fellow at Center for Genomic Science of IIT@SEMM, Campus Ifom-IEO, Milan.
Activity: Setup of a High Content Screening (HCS) based on Fluorescence Readouts.
01/06/05-16/02/12.
PhD in Molecular Biophysics at Center for Nanotechnology Innovation and National Enterprise for nanoScience and nanoTechnologies (NEST) of Scuola Normale Superiore of Pisa in collaboration with the Istituto Italiano di Tecnologia (IIT).
Grade Obtained: 70/70.
Thesis topic: A novel GFP-based sensor for combined measurements of pH and chloride in living cells.
Research Activity: Main project: Development of Nano-tools (bio-sensors) to monitor analytes alterations (such Cl-,pH) in real-time living cells . Side project: Drug-delivery and drug photorelease systems by metal Nanoparticles carrier in living cells.
Exams supported during PhD:
Photochemistry (Grade obtained: 30/30; Chemistry Department-University of Pisa)
Molecular Biophysics (Grade obtained: 28/30; Scuola Normale Superiore, Pisa).
Condensed Matter Structure (elements of quantum mechanics) (Grade obtained: 28/30; Scuola Normale Superiore, Pisa)
01/01/04-31/05/05.
Graduate Fellow in “Production and characterization of auto- fluorescent recombinant proteins” at Physics laboratory of Scuola Normale Superiore, Pisa.
May 2003-December 2003:
Stage in Molecular Biology; Laboratory of Gene and Molecular Therapy-CNR, Pisa.
July 2003.
Biologist Enabled-University of Pisa.
Grade obtained: 150/150.
06/05/2003.
Master Degree in Molecular Biology, specialization in Biotechnology- University of Pisa.
Grade obtained: 110 / 110 cum laude.
January 2001-December 2001.
Technical Assistant at Botanic Department-University of Pisa.
International Schools and Conferences:
• ISN-ESN, Athens, Greece, 28-1 September 2011: Oral presentation (in substitution of Prof. Beltram).
• Biophysical meeting, San Francisco,USA, February 2010: Poster presentation.
• 9rd International Summer School on Biophysics (Rovinj, Croatia, September 2006, 2 weeks).
• Time Resolved Spectroscopic Methods in Biophysics (Venice, Janury 6-Janury 20, 2006).
• 3rd European Course on Principles and Applications of Time-Resolved Fluorescence Spectroscopy (Berlin, Germany October 31-November 4, 2005).
• “Mutlidisciplinary aspects of Mass Spectroscopy” Dr. Karoly Vekey del Chemical Research Center, Hungarian Academy of Sciences. Pisa, 5-12 aprile 2005 - Dipartimento di Chimica – Università di Pisa.
Other Publications
Publications and Patents:
· Co-owner of European patent N. 081655227 (TO2007A000687): “Biosensore combinato di Cloro e pH”/ ”New GFP mutant sensitive to pH and concentration of chloride”
· Co-owner of Italian patent N. TO2011A000561: ” Vettore per il rilascio di un agente attivo, fotoscindibile per irraggiamento nel visibile”.
1. Single-step click-multifunctional coating for gold nanospheres. Valerio Voliani, Fernanda Ricci, Stefano Luin, and Fabio Beltram. IN SUBMISSION.
2. Smart Delivery and Controlled Drug Release with Gold Nanoparticles: New Frontiers in Nanomedicine. Valerio Voliani, Giovanni Signore, Riccardo Nifosí, Fernanda Ricci, Stefano Luin and Fabio Beltram. Recent Patents on Nanomedicine, 2012, 2 IN PRINT.
3. Multiphoton Molecular Photorelease in Click-Chemistry Functionalized Gold Nanoparticles. Valerio Voliani, Fernanda Ricci, Giovanni Signore, Riccardo Nifosì, Stefano Luin, and Fabio Beltram. Small 2011 Dec 2;7(23):3271-5.
4. Single-step coating and bifunctionalization of gold nanoparticles. Valerio Voliani, Stefano Luin, Fernanda Ricci and Fabio Beltram. Nanoscale. 2010 Dec 1; 2(12): 2783-9.
5. Simultaneous intracellular Cl- and pH measurement using a GFP-based sensor. Daniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi, Fabio Beltram. Nat Methods. 2010 Jul; 7(7):516-8.
6. Quantitative FRET analysis with the EGFP-mCherry fluorescent protein pair. Albertazzi L, Arosio D, Marchetti L, Ricci F, Beltram F. Photochem Photobiol. 2009 Jan-Feb;85(1):287-97. Epub 2008 Aug 27.
7. Green fluorescent protein ground states: the influence of a second protonation site near the chromophore. Bizzarri R, Nifosì R, Abbruzzetti S, Rocchia W, Guidi S, Arosio D, Garau G, Campanini B, Grandi E, Ricci F, Viappiani C, Beltram F. Biochemistry: 2007 May 8;46(18):5494-504. Epub 2007 Apr 17.
8. Spectroscopic and structural study of proton and halide ion cooperative binding to GFP. Arosio D, Garau G, Ricci F, Marchetti L, Bizzarri R, Nifosì R, Beltram F. Biophys J. 2007 Jul 1;93(1):232-44. Epub 2007 Apr 13.
9. Development of a novel GFP-based ratiometric excitation and emission pH indicator for intracellular studies. Bizzarri R, Arcangeli C, Arosio D, Ricci F, Faraci P, Cardarelli F, Beltram F. Biophys J. 2006 May 1;90(9):3300-14.
10. Resting smooth muscle cells as a model for studying vascular cell activation. Poliseno L, Cecchettini A, Mariani L, Evangelista M, Ricci F, Giorgi F, Citti L, Rainaldi G. Tissue Cell. 2006 Apr;38(2):111-20.
11. Two dimensional patterning of fluorescent proteins in hydrogels. Di Benedetto F, Biasco A, Bizzarri R, Arosio D, Ricci F, Beltram F, Cingolani R, Pisignano D. Langmuir. 2006 Jan 3;22(1):29-31.
12. Identification of active siRNAs against IGF-IR of porcine coronary smooth muscle cells in a heterologous cell line. Poliseno L, Evangelista M, Ricci F, Bonotti A, Nannipieri M, Rainaldi G. Int J Mol Med. 2005 Apr;15(4):713-8.
Fernanda Ricci
