Bio
Education
Bachelor of Science (First Class, Honors), University of Bath, UK (1997). Molecular and Cellular Biology.
Doctor of Philosophy, Imperial College, London, UK (2001): Virology and Cell Biology. Genotoxin-induced apoptosis in human lymphoid cells. Advisor: Professor Martin J Allday
Research and training
Postdoctoral Research Fellow, Wahl Lab, Salk Institute for Biological Studies, USA. (2001-2006). Focus on oncogene-induced DNA damage, and oncogenic suppression of p53 tumor suppressor activity. Author on 5 peer reviewed articles
Senior Research Associate, Wahl Lab, Salk Institute for Biological Studies, USA. (2007-March 2011). Development and validation of cell based screens to interrogate the p53 pathway and screen for p53-activating molecules. Author on 7 peer-reviewed articles.
Other Publications
Wade M, Li YC, Matani AS, Braun SM, Milanesi F, Rodewald LW, Wahl GM. Functional analysis and consequences of Mdm2 E3 ligase inhibition in human tumor cells. Oncogene 2012 Jan 23. doi: 10.1038/onc.2011.625. [Epub ahead of print]
Bernal F, Wade M, Godes M, Davis TN, Whitehead DG, Kung AL Wahl GM and Walensky LD (2010). A Stapled p53 Helix Overcomes HDMX-Mediated Suppression of p53. Cancer Cell 18(5):411-22.
Wade M, Wang YV and Wahl GM (2010). The p53 orchestra: mdm2 and mdmx set the tone. Trends in Cell Biology 20(5):299-309.
Wang YV, Wade M and Wahl GM (2009). Guarding the Guardian: Mdmx plays important roles in setting p53 basal activity and determining biological responses in vivo. Cell Cycle 6;8(21).
Wang YV, LeBlanc M, Wade M, Jochemsen A and Wahl GM (2009). Increased radio-resistance and accelerated B-cell lymphomas in mice with Mdmx mutations that prevent modifications by DNA damage-activated kinases. Cancer Cell Jul 7;16(1):33-43
Wade M and Wahl GM (2009). Targeting Mdm2 and Mdmx in cancer therapy: better living through medicinal chemistry? Mol Cancer Res 7: 1-11 (Most highly cited article in this journal for 2009)
Wade M, Rodewald LW, Espinosa JM and Wahl GM (2008). BH3 activation blocks Hdmx suppression of apoptosis and co-operates with Nutlin to induce cell death. Cell Cycle July 1:7;(13):1973-82.
Wang Y, Wade M, Wong ET, Li YC, Rodewald LW, and Wahl GM (2007). Quantitative analyses reveal the importance of regulated Hdmx degradation for P53 activation PNAS Jul 24;104(30):12365-70
Leao M, Anderton E, Wade M, Meekings K, and Allday MJ (2006). Epstein-Barr virus (EBV)-induced resistance to drugs that activate the mitotic spindle assembly checkpoint in Burkitt's lymphoma cells. J. Virol. 2007 Jan;81(1):248-60. Epub 2006 Oct 11
Wade M, Wong ET, Tang M, Stommel JM and Wahl GM (2006). Hdmx modulates the outcome of p53 activation in human tumor cells. J Biol Chem Nov 3;281(44):33036-44. Epub Aug 13.
Wade M and Wahl GM (2006). c-Myc, Genome Instability, and Tumorigenesis: The Devil is in the Details. In “The Myc/Max/Mad Transcription Factor Network”. Editor Robert Eisenman. Current Topics in Microbiology and Immunology Vol 302. pp169-203. Publisher: Springer-Verlag.
Wahl GM, Stommel JM, Krummel KA and Wade M (2005) Gatekeepers of the guardian: p53 regulation by post-translational modification, mdm2 and mdmx. In "25 Years of p53 Research". Chapter 4:73-113. Editors: Klas Wiman and Pierre Hainaut. Publisher: Springer-Verlag
Vafa O, Wade M, Kern S, Beeche M, Pandita TK, Hampton GM, Wahl GM (2002). c-Myc can induce DNA damage, increase reactive oxygen species, and mitigate p53 function: a mechanism for oncogene-induced genetic instability. Mol Cell 9(5):1031-44.
Wade M and Allday MJ (2000). Epstein-Barr virus abrogates a G2/M checkpoint activated by genotoxins. Mol Cell Biol. 2000 Feb;20(4):1344-60.
Walsh DA, Wade M, Mapp PI and Blake DR (1998). Focally regulated endothelial proliferation and cell death in human synovium. Am J Pathol 152(3):691-702
Projects
Screening Unit
My experience with developing and validating cell-based screening assays convinced me of their utility as discovery biology tools. In setting up the Screening Unit, I aimed to create an infrastructure with the flexibility to grow with our needs, and build a team that will contribute significantly to the design, development and execution of screening campaigns. The expertise present within our team facilitates the translation of ideas born on the lab bench into high-throughput homogenous and/or phenotypic assays. We are initially siRNA-focused, and are developing in-house projects in addition to collaborative efforts with other life science researchers.
Internal Projects
We have a strong basic research interest in proteins involved in ubiquitin signaling, with a particular focus on inflammation and cancer. Centered around a PhD program, we are integrating multiple approaches in order to uncover novel links between ubiquitin signaling and human pathologies. This strategy is aided by our screening environment, which enhances the types of discovery biology we can perform and provides a unique research environment for our team members.
Mark Wade
